Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development.

OBJECTIVE To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development. DESIGN Animal study. SETTING University laboratory. ANIMAL(S) CD-1 mice. INTERVENTION(S) Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 micromol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium. MAIN OUTCOME MEASURE(S) Oocyte maturation, fertilization, and early embryonic development in vitro. RESULT(S) After 6 and 24 hours of prematuration culture with 1 micromol/L, 5 micromol/L, and 10 micromol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls. CONCLUSION(S) Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.